Speaker 1
Development of Optical Microsphere Nanoscope: Fundamental Research to Technology Commercialization
光学微球纳米显微镜：从基础研究到技术产业化
Hong Minghui
洪明辉 教授
ABSTRACT
To push optical microscope resolution beyond 100 nm is revolutionary in the field of nano-imaging. It has been demonstrated that when a dielectric transparent microsphere at a diameter of a few microns is located on a sample surface, its imaging resolution can be enhanced greatly down to 50 nm, even 25 nm by coupling into a confocal microscope. However, the contact mode operation has a critical drawback that the relative location of microsphere and sample cannot be adjusted, also limiting its imaging field and contaminating sample surfaces. To address this issue, a non-contact scanning mode of optical microsphere nanoscope is successfully developed via special holder designs and flexible control of the microsphere movement. Feature sizes down to sub-50 nm can be easily observed. The commercialization progress to push OptoNano 200 (in the process to OptoNano 100) products into the market will also be reported.
将光学显微镜的分辨率推进到100纳米以下是光学纳米成像领域的革命性进步。我们之前的研究表明，直径为几微米的介质透明微球放置于样品表面上时，对于样品的成像分辨率可提升至50纳米左右，同激光扫描共聚焦显微镜进一步结合，更能观测到25纳米的尺度。然而，接触式成像模式有着显而易见的缺陷，如微球与样品的相对位置无法改变，视场极为受限，以及污染样品表面。为解决这一难题，基于一种特制的支架控制设计，我们开发出可灵活调控的非接触式扫描成像光学微球纳米显微镜。该设备可直接观察到小于50纳米的样品结构。在本报告中，我们还会谈到基于此技术的产业化进展，特别是如何将OptoNano 200这一产品（以及开发中的OptoNano 100）从研发推进到市场。
BIOGRAPHY
Prof. Hong Minghui specializes in laser microprocessing & nanofabrication, optical engineering and laser applications. He has co-authored 15 book chapters, 42 patents granted, ~ 500 scientific papers, and ~ 100 plenary/keynote/invited talks in international conferences. He is a member of organizing committees for Laser Precision Micromachining International Conference (2001~2021), International Symposium of Functional Materials (2005, 2007 and 2014), Chair of International Workshop of Plasmonics and Applications in Nanotechnologies (2006), Chair of Conference on Laser Ablation (2009) and Chair of Asia-Pacific Near-field Optics Conference (2013 and 2019). Prof. Hong is invited to serve as an Editor of Light: Science and Applications, Engineering, Science China G, Physics, Laser Micro/nanoengineering, and utive Editor-in-chief of Opto-Electronic Advances. Prof. Hong is Fellow of Academy of Engineering, Singapore (SAEng), Fellow of Optical Society of America (OSA), Fellow of International Society for Optics and Photonics (SPIE), Fellow of International Academy of Photonics and Laser Engineering (IAPLE) and Fellow of Institution of Engineers, Singapore (IES). Prof. Hong is currently a Full Professor and the Director of Advanced Research and Technology Innovation Centre, Director of Optical Science and Engineering Centre, Department of Electrical and Computer Engineering, National University of Singapore. He is also a founder of Phaos Technology Pte. Ltd.
洪明辉教授致力于激光微纳加工、光学工程和激光应用等领域的研究。他本科和硕士均毕业于厦门大学物理学系，2000年获新加坡国立大学博士学位，现任新加坡国立大学前沿研究和技术创新中心主任、光科学与工程中心主任、终身正教授和博士生导师。迄今为止已参与出版学术著作15本，获得授权专利42项，发表学术论文500余篇，受邀在国际学术会议上作报告100余次。曾在国际激光精密微加工大会（2001-2021年）和国际功能材料学术会议（2005、2007和2014年）担任组委会成员，2006年任国际等离子体及纳米技术应用论坛主席，2009年任激光烧蚀学术会议主席，2013和2019年任亚太近场光学国际会议主席。应邀担任《光：科学与应用》、《工程》、 《物理》、《中国科学：物理 力学 天文学》和《激光微纳工程》 等高水平学术期刊的编委，现任《光电进展》执行主编。洪明辉教授为美国光学学会会士、国际光学工程学会会士、国际光子学与激光工程学会会士、新加坡工程师协会会士，2018年当选为新加坡工程院院士。洪教授曾获“东盟杰出工程成就奖”、新加坡工程师学会“权威工程成就奖” 和教育部教育服务奖。洪教授也是新加坡光技术有限公司的创始人。
Speaker 2
Enabling Microfluidic Technologies for Recognition, Isolation and Single-Cell Analysis of Circulating Tumor Cells
基于微流控芯片肿瘤液体活检新方法
杨朝勇
Chaoyong Yang
ABSTRACT
Up to ninety percent of cancer-related deaths are caused by static cancer. Circulating tumor cells (CTCs), a type of cancer cells that spread through blood after detaching a solid tumor, are essential for the establishment of distant stasis for a given cancer. As a new type of liquid biopsy, analysis of CTCs offers the possibility to avoid invasive tissue biopsy procedures with practical implications for diagnostics. The fundamental challenges of analyzing and profiling CTCs are the extremely low abundances of CTCs in the blood and the intrinsic heterogeneity of CTCs. Various technologies have been proposed for the enrichment and single-cell analysis of CTCs. In this talk, I will present our microfluidic approaches for CTC analysis, ranging the specific ligand-aptamer evolution for CTC recognition, synergetic strategies for CTC isolation, to single-cell analysis of CTCs.
液体活检是通过对体液中循环肿瘤细胞（CTC）等靶标检测分析进行诊断的新技术，具有分子信息全、侵入性小、成本低等优势，已成为肿瘤诊断领域的前沿及研究热点。然而，循环靶标通常含量稀少、异质性强，且体液环境复杂。因此，亟需发展循环靶标高灵敏检测和高通量单细胞分析的液体活检新方法。近年来，基于微流控技术和机器学习，我们发展了核酸适体的高效筛选新方法，获得多条高亲和力、高特异性核酸适体序列，为液体活检提供理想的分子识别工具；结合流体调控与微纳界面仿生多价组装，我们构筑了基于细胞尺寸与生物识别特性协同捕获的微流控微柱阵列芯片，实现了循环肿瘤细胞的高效捕获与无损释放；借助微流体器件的精准操控优势，我们开发了一系列高通量单细胞分析方法，用于揭示循环肿瘤细胞的分子病理信息。所发展的识别探针、捕获芯片与高通量单细胞分析方法极大地推动了液体活检技术的发展，在癌症的精准诊断、用药指导、疗效评估方面具有重要的临床应用前景。
BIOGRAPHY
Professor Chaoyong Yang is a Distinguished Professor in the Department of Chemical Biology, Xiamen University. He received his PhD University of Florida under the guidance of Professor Weihong Tan. After postdoc training in Professor Richard Mathies’ group at UC Berkeley, he joined Xiamen University in 2008. Professor Yang has published more than 220 research papers in international journals which have received over 12,000 citations. He coauthored 2 books and holds 45 patents. He is the associate editor of ACS Applied Bio Materials and has served in the advisory board of 10 international journals. He is a Fellow of the Royal Society of Chemistry and senior member of Chinese Chemical Society. He received many awards including CAPA Distinguished Faculty Award in 2012, National Outstanding Young Investigator Award in 2013, Chinese Young Analyst Award in 2015, Chinese Chemical Society-Royal Society of Chemistry Young Chemist Award in 2016, and 2021 ACS Advances in Measurement Science Lectureship Award. His research focuses on the development of novel chemical and microfluidic tools for life science research and disease diagnosis, which integrates molecular engineering approaches, bio-nanotechnologies, and microfluidics to develop novel methods, smart materials, and functional miniaturized devices to address previously-intractable bioanalytical and biomedical problems.
杨朝勇教授是国家杰出青年科学基金获得者、厦门大学南强特聘教授、谱学分析与仪器教育部重点实验室副主任、中国化学会化学生物学专业委员会副主任、英国皇家化学会Fellow、美国化学会ACS Applied Bio Materials副主编。杨朝勇教授分别从厦门大学、美国佛罗里达大学获得本科和博士学位，2006-2007在美国加州大学伯克利分校从事博士后研究。2008年回到厦门大学任教。他长期致力于生物分析化学研究，在微流控、单细胞单分子分析等方向取得了创新性的成果。在Chem Rev、Nature Commun、Sci Advance、Acc Chem Res、PNAS、JACS、Angew Chem Int Ed等学术刊物上发表论文220篇，中英文专著各1部，论文被引超过12000次，H指数58。获授权专利45项。先后获得中美化学与化学生物学教授协会杰出教授奖、中国青年分析化学家奖、中国化学会-英国皇家化学会青年化学奖、美国化学会测量科学进展讲座奖等奖项与荣誉。