Acoustofluidics: merging acoustics and microfluidics for biomedical applications
声流控：为生物医学应用融合了声学和微流控
Tony Jun Huang（黄俊）
ABSRACT
The past two decades have witnessed an explosion in lab-on-a-chip research with applications in biology, chemistry, and medicine. The continuous fusion of novel properties of physics into microfluidic environments has enabled the rapid development of this field. Recently, a new lab-on-a-chip frontier has emerged, joining acoustics with microfluidics, termed acoustofluidics. Here we summarize our recent progress in this exciting field and show the depth and breadth of acoustofluidic tools for biomedical applications through many unique examples, from exosome separation to cell-cell communications to 3D bioprinting, from circulating tumor cell isolation and detection to ultra-high-throughput blood cell separation for therapeutics, from high-precision micro-flow cytometry to portable yet powerful fluid manipulation systems. These acoustofluidic technologies are capable of delivering high-precision, high-throughput, and high-efficiency cell/particle/fluid manipulation in a simple, inexpensive, cell-phone-sized device. More importantly, the acoustic power intensity and frequency used in these acoustofluidic devices are in a similar range as those used in ultrasonic imaging, which has proven to be extremely safe for health monitoring during various stages of pregnancy. As a result, these methods are extremely biocompatible; i.e., cells and other biospecimen can maintain their natural states without any adverse effects from the acoustic manipulation process. With these unique advantages, acoustofluidic technologies meet a crucial need for highly accurate and amenable disease diagnosis (e.g., early cancer detection and monitoring of prenatal health) as well as effective therapy (e.g., transfusion and immunotherapy).
过去二十年见证了芯片实验室技术及其应用在生物学、化学和医学领域的爆炸式发展。物理新特性在微流控环境中的不断融合，使得这一领域得以迅速发展。最近，出现了一种结合了声学和微流控的新型芯片实验室前沿技术，称为声流控。在这里，我们总结了本研究团队在这个令人兴奋的领域的最新进展，并且通过许多独特的例子显示了声流控工具在生物医学应用方面的深度和广度，从外泌体分离到细胞与细胞间的信息通信到生物3D打印，从循环肿瘤细胞的分离与检测到用于治疗的超高通量的血细胞分离,从高精度的微流血细胞计数到便携但强大的流体操控系统。这些声流控技术能够在简单、廉价、手机大小的设备中提供高精度、高通量和高效率的细胞/粒子/流体操控。更重要的是，这些声流控设备中使用的声功率强度和频率与已被证明是非常安全的、可以在怀孕的各个阶段进行健康监测的超声波成像设备的声功率强度和频率相近。因此，这些方法具有极强的生物相容性；即，细胞和其他生物样本可以保持它们的自然状态，而不会受到声学操控过程中的任何负面影响。声流控技术以其独特的优势，满足了疾病诊断（如早期癌症检测和产前健康监测）和有效治疗（如输血和免疫治疗）的关键需求。
BIOGRAPHY
Tony Jun Huang is the William Bevan Distinguished Professor of Mechanical Engineering and Materials Science at Duke University. Previously he was a professor and the Huck Distinguished Chair in Bioengineering Science and Mechanics at The Pennsylvania State University. He received his Ph.D. degree in Mechanical and Aerospace Engineering from the University of California, Los Angeles (UCLA) in 2005. His research interests are in the fields of acoustofluidics, optofluidics, and micro/nano systems for biomedical diagnostics and therapeutics. He has authored/co-authored over 220 peer-reviewed journal publications in these fields. His journal articles have been cited more than 17,000 times, as documented at Google Scholar (h-index: 72). He also has 26 issued or pending patents. He was elected a fellow of the following six professional societies: the American Association for the Advancement of Science (AAAS), the American Institute for Medical and Biological Engineering (AIMBE), the American Society of Mechanical Engineers (ASME), the Institute of Electrical and Electronics Engineers (IEEE), the Institute of Physics (IoP), and the Royal Society of Chemistry (RSC). Huang’s research has gained international recognition through numerous prestigious awards and honors including a 2010 National Institutes of Health (NIH) Director’s New Innovator Award, a 2012 Outstanding Young Manufacturing Engineer Award from the Society for Manufacturing Engineering, a 2013 American Asthma Foundation (AAF) Scholar Award, JALA Top Ten Breakthroughs of the Year Award in 2011, 2013, and 2016, the 2014 IEEE Sensors Council Technical Achievement Award from the Institute of Electrical and Electronics Engineers (IEEE), the 2017 Analytical Chemistry Young Innovator Award from the American Chemical Society (ACS), the 2019 Van Mow Medal from the American Society of Mechanical Engineers (ASME), and the 2019 Technical Achievement Award from the IEEE Engineering in Medicine and Biology Society (EMBS).
黄俊（Tony Jun Huang）博士是杜克大学机械工程与材料科学系William Bevan杰出讲席教授。此前，他是宾州州立大学生物工程科学与力学系的Huck杰出讲席教授。2005年在加州大学洛杉矶分校机械与航空工程系获得博士学位。他的研究兴趣在声流控、光流控、和微/纳系统在生物医药诊断和治疗领域的应用研究。到目前为止，他在这些领域发表了超过220篇同行评审的期刊论文。他的期刊论文总引用次数超过17000次，谷歌H因子达到72。并且，他还拥有26项授权/在审专利。他已被选为以下六个国际专业学会的会士（fellow）：美国科学促进会（AAAS）、美国医学和生物工程学会（AIMBE）、美国机械工程师协会（ASME）、国际电气和电子工程师协会（IEEE）、英国物理学会（IOP）、英国皇家化学学会（RSC）。同时，他的研究也获得了诸多的奖项和荣誉，其中包括2010年美国国立卫生研究院（NIH）青年创新奖，2012年国际制造工程学会杰出青年制造工程师奖，2013年美国哮喘基金会（AAF）学者奖，2011、2013、2016年JALA年度最佳十大突破奖，2014年国际电气和电子工程师协会（IEEE）的传感器理事会技术成就奖（IEEE），2017年美国化学学会（ACS）分析化学青年创新奖，2019年美国机械工程师协会（ASME）Van Mow奖章和2019年IEEE EMBS技术成就奖。